Document Details
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Document Type |
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Article In Journal |
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Document Title |
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Genotype and antiretroviral drug resistance of human immunodeficiency virus-1 in Saudi Arabia مقاومة فيروس نقص المناعة المكتسب لمضادات الفيروسات القهقرية وتحديد التنوع الجيني للفيروس لدى المرضى السعوديين |
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Subject |
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HIV |
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Document Language |
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English |
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Abstract |
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Objectives: To analyze antiretroviral drug resistance and
determine the genotype of human immunodeficiency
virus (HIV)-1 in Saudi patients by sequencing an
amplified region of the viral pol gene.
Methods: This retrospective study analyzed data from
plasma samples submitted for genotypicdrug sensitivity
monitoring. Samples were analyzed at the Special
Infectious Agent Unit, King Fahd Medical Research
Center of King Abdulaziz University, Jeddah from
August 2004 to June 2009. The Viroseq2.5® kit (Celera/
Abbott) was used with ABI Prism 3100 sequencer. All
patients were Saudi nationals and were on antiretroviral
therapy, some experiencing treatment failure.
Results: Based on protease region (PR), genotypes of 63
samples were as follows: C:22, G:21, B:9, CRF02_AG:5,
D:3, A:1, F:1, and J:1. Based on reverse transcriptase
region (RT), genotypes were as follows: C:23, G:24,
B:9, CRF02_AG: 2, D:2, A:1, and F:1. Antiretroviral
susceptibility testing results were as follows: 52% of
the isolates were susceptible to all 3 major classes of
antiretroviral drugs used, 41% had mutations known
to confer high level resistance to one or more of the
nucleoside analogue reverse transcriptase inhibitors, 16%
had mutations known to confer high level resistance
to non-nucleoside analogues reverse transcriptase
inhibitors, 13% had mutations known to confer high
level resistance to one or more of the protease inhibitors
(PI). Most isolates were susceptible to 2 or at least one
class of antiretroviral, and only 3% of the isolates had
resistance to several members of all 3 classes.
Conclusion: Antiretroviral resistance is not uncommon
in Saudi patients on antiretroviral therapy. |
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ISSN |
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0 |
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Journal Name |
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Saudi Medical Journal |
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Volume |
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31 |
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Issue Number |
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9 |
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Publishing Year |
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1431 AH
2010 AD |
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Article Type |
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Article |
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Added Date |
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Tuesday, November 9, 2010 |
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Researchers
| غازي عبداللطيف جمجوم | jamjoom, Ghazi abdullatif | Investigator | Doctorate | gjamjoom1@yahoo.com |
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Files
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27917.pdf
| pdf | مقالة علمية لكرسي الأيدز |
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